DHM microscope – Life science
DHM T Serie
2. Study unexplored biological processes
3. Perform multimodal imaging
4. Optical profilometer configured for transmission
5. Study innovative materials and devices
Transmission DHM® measures the difference in optical path of a beam passing through samples.
For measurements of micro-optical components, microfluidic devices and defects or particles within transparent samples, Transmission DHM® systems are generally the optimal choice.
In biological imaging, the pioneering users of DHM® have demonstrated, through major publications, the interpretation of DHM® measurements in terms of underlying biological processes. DHM® transmission systems are the best choice for such cellular applications.
Two DHM® Transmission configurations are available, differentiated by the number of wavelengths.
- The T1000 models are configured with a single wavelength and are the ideal tool for studying simple living cells.
as measurement of transparent material samples with smooth surfaces - T2100 models are configured to perform simultaneous measurements at two wavelengths, thereby increasing measurement capacity
Applications
Living cells
- Cell-based tests
- Ionic channels
- 4D cell imaging
- Red cells
- Electro-neutral ion transport
- Water channels
More info on applications
- Surface topography
- Surface finish
- Fault inspection
- Structured thin film
- MEMS measurement
3D profilometry at unmatched speed
DHM® allows non-toxic quantitative measurements of individual living cells and cultures up to confluence:
- Time lapse
- Screening of multi-well plates
- diagnostic
Study unexplored biological processes
The quantitative phase measurement of DHM® can be interpreted in terms of many underlying biological processes:
- chain activity
- Cell viability
- intracellular concentration
- morphological changes
Perform multimodal imaging
An optional module allows simultaneous DHM® and fluorescence measurements:
- Correlate DHM® measurements
with well-known measurement protocols
- Use DHM to decrease the number
fluorescence marking required
- improve understanding of the cellular mechanism
Optical profilometer configured for transmission
The DHM® transmission makes it possible to measure several sample characteristics:
- optical topography sample
- Thickness variability
- Size and location of internal structures and faults
- Refractive index and concentration
- birefringence
Study innovative materials and devices
The advantages of rapid dynamic measurement have been demonstrated in many applications:
- Microoptical
- Microfluidic
- Stress and stress analysis
- Liquid crystal displays (LCD)
- biophysics
- Wettability of coatings and structures
- Fluid and gas dynamics
- Dissolution and crystallization
- Particle velocimetry
Time sequence of the 3D optical map of the quantitative phase measurement, limited by the camera rate: division of the HeLa cells
ADVANTAGES
- Non invasive
- Study unexplored biological processes
- Perform multimodal imagery
- Optical profilometer configured for transmission
- Study innovative materials and devices
Spécifications
T serie specifications
| T1000 | T2100 | |
| Configuration | One laser source | Two laser sources |
| Measurement modes | Single wavelength | Single and dual wavelength |
| Accuracy (as demonstrated by taking the temporal standard deviation on 1 pixel over 30 measurements) | 1.0 nm 1 | 1.0 / 5.0 nm 1 * |
| Vertical resolution (defined as twice the accuracy) | 2.0 nm 1 | 2.0 / 10.0 nm 1 * |
| Repeatability (as demonstrated by taking the one sigma Rq value of 30 repeatability measurements on SiC reference mirror) | 0.02 nm 1 | 0.02 / 0.05 nm 1 * |
| Vertical measuring range (without any scanning) |
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| Max. height of steps with sharp edges (Depends on the laser source(s) and operating wavelength(s) |
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1) Converted value for measurements in air and with sample refractive index n=1.5 2) Converted value for measurements in water and with sample refractive index n=1.5 * With / Without single wavelength mapping
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